Following my PhD and postdoctoral appointments I joined GlaxosmithKline as a Senior Scientist within Drug Discovery. Over 20 years I held various positions within Drug Discovery that include a post as Team Leader at Principal Scientist level. During my tenure at GSK I received a number of science awards and in 2008 was awarded an exceptional science award for my work in area of Neuroscience.
In 2010 I joined Middlesex University to take on a post as Reader in Bioanalytical Science. The position involves teaching, supervision, research and consultancy work. The research areas that I am involved in are development of platform for metabolomics, application of analytical techniques like mass spectrometry for measurement of molecules in biological matrices and development/improvement of analytical instruments in collaboration with instrument manufacturers.
Publications
Shah, A. J.; Adlard, M. W. Trace level measurement of b-lactam antibiotics and biosynthetic intermediates following precolumn derivatisation with 9-fluoroenylmethylcholorformate. J. Chromatog. (1988) 325-336.
Shah, A. J.; Adlard, M. W.; Holt, G. Quantitation of natural penicillins in fermentation media by high performance liquid chromatography using precolumn derivatisation with 1-hydroxybenzo-triazole. Analyst (1988) 1197-200.
Shah, A. J.; Adlard, M. W.; Stride, J. D. A sensitive assay for clavulanic acid and sulbactam in biological fluids by high performance liquid chromatography and precolumn derivatisation. J. Pharm. Biomed. Anal. (1990) 437-443.
Shah, A. J.; Tilburn, J.; Adlard, M. W.; Arst, H. N., Jr. pH Regulation of penicillin production in Aspergillus nidulans.FEMS Microbiol. Letts. (1991) 209-212.
Shah, A. J.; Younie, D. A.; Adlard, M. W.; Evans, C. S. High-performance liquid chromatography of non-protein amino acids produced in Acacia seeds. Phytochem. Anal. (1992) 20-25.
Evans, C. S.; Shah, A. J.; Adlard, M.W.; Arce, M. L. R. Non-protein amino acids in seeds of neotropical species of Acacia. Phytochemistry (1992) 123-126.
Shah A.J; Orford C D.; Adlard M. W. Derivatisation methods for trace-level quantification of b-lactam antibiotics. LC-GC (1993).
Shah, A. J.; Younie, D. A.; Adlard, M. W.; Evans, C. S. Evolutionary trends within genus Acacia based on the accumulation of non-protein amino acids in seeds. Advance in legume Systematics 5 (1994).
Shah, A.; de Biasi, V.; Camilleri, P. Development of a spectrophotometric method for the measurement of thiols at trace levels. Anal. Proceedings (1995) 149-153.
Shah, A; Rahman S.S; Biasi V. de; Camilleri P; Development of a colorimetric method for the detection of amines bound to solid support. (1995) 149-153.
Hagan, J. J.; Leslie, R. A.; Patel, S.; Evans, M. L.; Wattam, T. A.; Holmes, S.; Benham, C. D.; Taylor, S. G.; Routledge, C.; Hemmati, P.; Munton, R. P.; Ashmeade, T. E.; Shah, A. J.; Hatcher, J. P.; Hatcher, P. D.; Jones, D. N. C.; Smith, M. I.; Piper, D. C.; Hunter, A. J.; Porter, R. A.; Upton, N. CNS Localisation and Effects of Orexin-A.Proceedings of the National Academy of Sciences of the United States of America (1999) 10911-10916.
Shah, A. J.; de Biasi, V.; Taylor, S. G.; Roberts, C.; Hemmati, P.; Munton, R.; West, A.; Routledge, C.; Camilleri, P. Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. J. Chrom. B: Biomedical Sciences and Applications (1999) 133-140
Hadley M; Gilges M; Senior J; Shah A; Camilleri; Capillary Electrophoresis in the pharmaceutical industry:applications in discovery and chemical development.J. Chrom. B: Biomedical Sciences and Applications (1999) 177-188.
Hemmati, P.; Shilliam, C. S.; Hughes, Z. A.; Shah, A. J.; Roberts, J. C.; Atkins, A. R.; Hunter, A. J.; Heidbreder, C. A. In vivo characterization of basal chemoneuroanatomical markers for amino acids in the shell and core subterritories of the nucleus accumbens. Neurochemistry International (2001) 199-208.
Heidbreder, C. A.; Lacroix, L.; Atkins, A. R.; Organ, A. J.; Murray, S.; West, A.; Shah, A. J. Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain. Journal of Neuroscience Methods (2001) 135-144.
Senior J; Shah A; Monteux C; Biasi V De; The evaluation of interdigitated array electrodes for the measurement of catecholamines and indoleamines. J. Pharm. Biomed. Anal. (2001) 843-848.
Tolson, D.; Organ, A.; Shah, A. Development of a high-pressure gradient pumping system for parallel liquid chromatography/mass spectrometry for the analysis of combinatorial libraries. Rapid Comm. Mass Spectro. (2001) 1244-1249.
Heidbreder, C. A.; Foxton, R.; Cilia, J.; Hughes, Z. A.; Shah, A. J.; Atkins, A.; Hunter, A. J.; Hagan, J. J.; Jones, Declan N. C. Increased responsiveness of dopamine to atypical, but not typical antipsychotics in the medial prefrontal cortex of rats reared in isolation. Psychopharmacology (2001) 338-351
Hows, M, E. P.; Organ, A. J.; Murray, S.; Dawson, L. A.; Foxton, R.; Heidbreder, C.; Hughes, Z. A.; Lacroix, L.; Shah, A. J. High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates. J. Neuroscience Methods (2002) 33-39.
Shah, A. J.; Crespi, F.; Heidbreder, C. Amino acid neurotransmitters: separation approaches and diagnostic value. J. Chromatog. B: Analytical Technologies in the Biomedical and Life Sciences (2002) 151-163.
Lacroix, L. P.; Hows, M. E. P.; Shah, A. J.; Hagan, J. J.; Heidbreder, C. A. Selective Antagonism at Dopamine D3 Receptors Enhances Monoaminergic and Cholinergic Neurotransmission in the Rat Anterior Cingulate Cortex. Neuropsychopharmacology (2003) 839-849.
Dawson, L. A.; Organ, A. J.; Winter, P.; Lacroix, L. P.; Shilliam, C. S.; Heidbreder, C.; Shah, A. J. Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns. J. Chromatog. B: Analytical Technologies in the Biomedical and Life Sciences (2004) 235-241.
Hows, M. E. P.; Lacroix, L.; Heidbreder, C.; Organ, A.J.; Shah, A. J. High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. J. Neuroscience Methods (2004) 123-132.
Hows, M. E. P.; Ashmeade, T. E.; Billinton, A.; Perren, M. J.; Austin, A. A.; Virley, D. J.; Organ, A. J.; Shah, A. J. High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates. J. of Neuroscience Methods (2004) 221-226.
Shah, A. J.; De la Flor, R.; Atkins, A.; Dawson, L. A. Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N- acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. J. Chromatogr. B: Analytical Technologies in the Biomedical and Life Sciences (2008) 153-158.
Bianchi, M.; Shah A.J.; Fone, K.C.; Atkins, A.R.; Dawson, L.A.; Heidbreder, C.A.; Hows, M.E.; Hagan, J.J.; Marsden, C.A. Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus. Synapse (2009) 63 359-364.
Bianchi, M.; Fone, K.C.F.; Shah, A.J.; Atkins, A.R.; Dawson, L.A.; Heidbreder, C.A.; Hows, M.E.; Hagan, J.J.; Marsden, C.A.; (2009); Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats; European Neuropsychopharmacology: The Journal of the European College of Neuropsychopharmacology; 19; 778-790.
Fricker A-C, Selina M.H., de la Flor R., Shah A.J., Woolley M, Dawson L.A., Kew J. N.C. Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR. Neuropharmacology (2009) 56 1060-1067.
Di Daniel E.; Mok, M.H.S.; Mead, E.; Mutinelli, C.; Zambello, E.; Caberlotto, L.L.; Pell, T.J.; Langmead, C.J.; Shah, A.J.; Duddy, G.; Kew, J.N.C.; Maycox, P.R.; (2009); Evaluation of expression and function of the H+/myo-inositol transporter HMIT; BMC Cell Biology; 10; 1-12.
Trivedi, D; Jones, H, Shah, A.J., Iles, R.K; (2012) Development of zwitterionic hydrophilic liquid chromatography (ZIC-HILIC-MS) metabolomics method for shotgun analysis of human urine; 3; 144-156.
Serafim V., Shah A., Puiu M., Andreescu N., Coricovac D., Nosyrev A., Spandidos D.A., Tsatsakis A. M., Dehelean C., Pinzaru L. (2017) Classification of cancer cell lines using matrix-assisted laser desorption/ionization time‑of‑flight mass spectrometry and statistical analysis. Int. J. Mol. Med. 40(4) 1096-1104.
Shah, A J.; Shah, H N. (2017) Determination of Antimicrobial Resistance using Tandem Mass Spectrometry In: The Triumph of MALDI-TOF Mass Spectrometry and New Developments in Tandem Mass Spectrometry for Clinical Microbiology, 2nd London, Wiley.
Olkun A.; Shah, A J.; Shah, H N. (2017) Processing MALDI-TOF MS Data through BioNumerics for delineating intraspecies diversity In: The Triumph of MALDI-TOF Mass Spectrometry and New Developments in Tandem Mass Spectrometry for Clinical Microbiology", 2nd London, Wiley.
Serafim V.; Shah H.N.; Hennessy N.; Allen D.J.; Ring C.; Munoz L.P.; Shah A.J.; (2017) Mass Spectrometry Application in Viral Analysis In: The Triumph of MALDI-TOF Mass Spectrometry and New Developments in Tandem Mass Spectrometry for Clinical Microbiology", 2nd London, Wiley.
Serafim V., Ring C., Pantoja, L, Shah H, Shah A; (2017) Rapid Identification of E. coliBacteriophages using Mass Spectrometry. Journal of Proteomics and Enzymology. 2017, 5:2.
Antia I.U. , Yagnik D.R., Shah A.J., Hills F.A. (2017) Measurement of heparan sulphate disaccharides in biological samples using pre-column derivatisation with UPLC-MS and single ion monitoring. Anal. Biochem. 530, 17-30.
Wang S.X., Acha D., Shah
Gujarati
Hindi
Currently I teach at both undergraduate and postgraduate level.
BIO1601 - 'Elements of Life'
BMS4147 - 'Bioanalysis and Toxicology'
Lead BMS4977 - 'Advanced Bioanalytical Techniques' on the postgraduate programme.
My research interests include:
Shah, Ajit J. and Shah, Haroun N. and Gharbia, Saheer E. and Tranfield, Erika and Thompson, Clive , eds. (2023) Microbiological identification using MALDI-TOF and tandem mass spectrometry: industrial and environmental applications. Wiley. ISBN 978-1-119-81405-4 (Accepted/In press)
Shah, Ajit J. and Shah, Haroun N. and Duncan, Louise and Ward, Malcom (2023) Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa. In: Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications. Shah, Haroun N. and Gharbia, Saheer E. and Shah, Ajit and Tranfield, Erika and Clive, Thompson , eds. Wiley, pp. 183-201. ISBN 978-1-119-81405-4 (Accepted/In press)
Shah, Ajit J. and Shah, Haroun N. and Gharbia, Saheer E. and Francese, Simona (2023) Progress in the microbiological applications of mass spectrometry: From electron impact to soft ionization techniques, MALDI-TOF MS and beyond. In: Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications. Shah, Haroun N. and Gharbia, Saheer E. and Shah, Ajit and Tranfield, Erika and Clive, Thompson , eds. Wiley, pp. 1-35. ISBN 978-1-119-81405-4 (Accepted/In press)
Rudra, Bashudev and Duncan, Louise and Shah, Ajit J. and Shah, Haroun N. and Gupta, Radhey S. (2022) Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov. International Journal of Systematic and Evolutionary Microbiology , 72 (11). pp. 1-15. ISSN 1466-5026
Yagnik, Darshna and Ward, Malcolm and Shah, Ajit J. (2021) Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli. Scientific Reports , 11 (1). pp. 1-7. ISSN 2045-2322
